All of the following are nucleic acid amplification techniques EXCEPT?

Nucleic acid amplification techniques are methods used to increase the number of copies of specific DNA or RNA sequences, allowing for their detection and analysis. Techniques such as Reverse transcription PCR, Real-time PCR, and Nested PCR specifically deal with the amplification of nucleic acids.

Reverse transcription PCR begins by transcribing RNA into complementary DNA (cDNA), which is then amplified through the PCR process. Real-time PCR, also known as quantitative PCR (qPCR), allows for the monitoring of the amplification process in real time, providing quantitative data on the nucleic acid present. Nested PCR involves two rounds of amplification using two sets of primers, enhancing specificity and sensitivity.

In contrast, flow cytometry is a technique primarily used for analyzing the physical and chemical characteristics of particles in a fluid as they pass through at least one laser. While flow cytometry can incorporate molecular techniques, it does not involve the amplification of nucleic acids, making it the correct answer for this question. This method is focused more on the measurement and analysis of cellular properties rather than the amplification of genetic material.