How should mycobacterial cultures be incubated to promote growth?

Mycobacterial cultures are best incubated in an atmosphere enriched with 5% carbon dioxide. This environment helps to create optimal growth conditions for Mycobacterium species, including Mycobacterium tuberculosis, which is a slow-growing organism. The addition of carbon dioxide can enhance the growth of mycobacteria by improving the pH and providing a microenvironment that mimics the conditions found in the human body.

The slow growth rate of mycobacteria necessitates a longer incubation period compared to many other bacteria, often requiring several weeks. Using a CO2-enriched atmosphere helps maintain a stable environment conducive to their growth. Thus, incubating mycobacterial cultures in 5% CO2 significantly supports their viability and culture success.