Which diagnostic technique is used to detect tissue roundworms through muscle biopsy?

The technique specifically utilized to detect tissue roundworms, such as Trichinella spiralis, through muscle biopsy is indeed molecular techniques, particularly when you're looking for accurate identification of the organism at a genetic level. In cases like trichinosis, where the larvae invade muscle tissue, a muscle biopsy can isolate the larvae or larvae DNA directly from the tissue.

Histopathology is relevant in examining tissue sections under the microscope, which allows for identifying organisms based on their morphology and distribution. However, while histopathology is crucial for many diagnoses, it does not directly engage in the specific molecular identification that helps identify tissue roundworm infections like those caused by Trichinella.

Serological testing detects antibodies against these parasites, which can confirm exposure but does not provide direct evidence of the presence of the worms within muscle tissue itself.

Molecular techniques typically involve the use of polymerase chain reaction (PCR) or other similar methods for the detection of genetic material. They provide high specificity and sensitivity for identifying not just Trichinella spiralis but several other infectious pathogens when analyzing tissue samples.

In summary, for directly locating tissue roundworms from a muscle biopsy, the approach that would yield the necessary precision is often via molecular techniques, making it the correct choice